Trichosporon pullulans phadia setting the standard. Not the best method to use because of the yeastlike texture of the colony. An trichosporon asahii in uska species han fungi in nahilalakip ha divisio nga basidiomycota, ngan nga ginhulagway ni akagi, sugita, a. The aim of this study was to determine experimental conditions for in vitro biofilm formation of clinical isolates of trichosporon inkin, an important opportunistic pathogen in immunocompromised patients. Trichosporon asahii is an opportunistic fungus that causes infections in immunosuppressed patients. Aug 21, 2014 trichosporon asahii edge of a colony as viewed using the adhesive tape method. A rare case of recurrent urinary tract infection due to. Full text a fatal case of trichosporon asahii fungemia and. Sep 01, 2001 trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen seen particularly in immunologically compromised patients. Trichosporon asahii genotypes determined on the basis of.
Trichosporon asahii was the most frequent species detected. All species of trichosporon are yeasts with no known teleomorphs sexual states. Trichosporon inkin as an emergent pathogen in patients. Trichosporon asahii and trichosporon asteroides from plos. Integrated transcriptomic analysis of trichosporon asahii. Trichosporon beigelii is a minor component of normal skin flora, and is widely distributed in nature, but nevertheless is the most significant pathogen in the genus trichosporon. To the best of our knowledge this is the first report of a urinary tract infection caused by trichosporon asahii from western india. Case reportinvasive trichosporonosis due to trichosporon asahii. Successful treatment of breakthrough trichosporon asahii. Pdf disseminated, fatal trichosporon asahii infection in. Trichosporon asahii among intensive care unit patients at. Cog analysis predicted that 3,1 genes were assigned to 23 functional categories and that 628. Trichosporon inkin as an emergent pathogen in patients with. Neutropenia developing due to malignancies is an important risk factor for fungal infection.
Species distribution, trichosporon asahii genotypes determined on the basis of ribosomal dna. Trichosporon species are ubiquitously spread and known to be part of the normal human flora of the skin and gastrointestinal tract. Antachopoulos c, papakonstantinou e, dotis j, bibashi e, tamiolaki m, koliouskas d. A rare case of disseminated trichosporon asahii infection. Methodology fungal culture trichosporon asahii is currently the most common cause of invasive trichosporonosis 4, 18. Antifungal activities of tacrolimus in combination with antifungal agents against fluconazolesusceptible and fluconazoleresistant trichosporon asahii isolates. Trichosporon asahii is an emerging, lifethreatening opportunistic pathogen. Trichosporon asahii formerly known as trichosporon beigelii and cutaneum is a basidiomycetous yeast.
Trichosporon definition of trichosporon by medical. Trichosporon asahii is a major causative agent of deepseated trichosporonosis, which has a high mortality rate. Trichosporon asahii an overview sciencedirect topics. Scalp white piedra caused by trichosporon asahii maouni safae, lakhmiri myriam, senouci karima, meziane mariam department of dermatology, ibn sina hospital, rabat, morocco clinical image a 10yearold girl presented for asymptomatic whitish particles in. We present two pediatric oncology cases successfully treated with voriconazole after t. Fungemia due to trichosporon asahii in a neutropenic child refractory to amphotericin b. Here we describe trichosporon species distribution and prevalence of. After removal of the catheter, remission of the fever and a reduction in inflammatory markers was achieved. Suppurative flexor tenosynovitis from tsumakurella and trichosporon. Infeccion urinaria nosocomial por trichosporon asahii. A fatal case of trichosporon asahii fungemia and pneumonia in a kidney transplant recipient during caspofungin treatment meifang yang,1,2 hainv gao,1,2 lanjuan li1,2 1state key laboratory for diagnosis and treatment of infectious diseases, the first affiliated hospital, school of medicine, zhejiang university. Cell surface hydrophobicity and colony morphology of. Identification of trichosporon asahii by pcr based on.
Biofilms were formed in microtitre plates in three different media rpmi, sabouraud and cled, with inocula of 104, 105 or 106 cells ml. Trichosporon asahii, a noncandida yeast that caused fatal. Health, general antifungal agents analysis antiparasitic agents tacrolimus. However, hypersensitivity pneumonitis induced by 2 members of the trichosporon species, t. Januarymarch 2007 rastogi et al invasive trichosporonosis in nonimmunocompromised host trichosporonosis with trichosporon asahii. These yeast grow out forming hyphae or pseudohyphae, fragmenting into. Antifungal activities of tacrolimus in combination with. Trichosporonosis definition of trichosporonosis by medical. Trichosporon asahii trichosporon beigelii infections are rare but have been associated with a wide spectrum of clinical manifestations, ranging from superficial involvement in immunocompetent individuals to severe systemic disease in immunocompromised patients. Proliferation of trichosporon yeasts in the hair can lead to an unpleasant but nonserious condition. Bloodstream infection in a noncancer patient receiving combination antifungal therapy. Researcharticle rapid and simple detection of trichosporon asahii by optimized colony pcr dequanzhang,1,2 xuelianlu,3 yongliao,2 zhikuanxia,2 zhuoyingpeng,2 xinyang,2 androngyayang 2 armymedicaluniversityirdmilitarymedicaluniversity,chongqing,china. There are now 100 reported cases of hematogenously disseminated infections with this lifethreatening yeast, and no effective antifungal therapy is available.
Most are typically isolated from soil, but several species occur as a natural part of the skin microbiota of humans and other animals. Trichosporon asahii is a rare cause of disseminated fungal infection in immunocompromised patients, presenting a diagnostic and therapeutic challenge. May 06, 2014 a fatal case of trichosporon asahii fungemia and pneumonia in a kidney transplant recipient during caspofungin treatment meifang yang,1,2 hainv gao,1,2 lanjuan li1,2 1state key laboratory for diagnosis and treatment of infectious diseases, the first affiliated hospital, school of medicine, zhejiang university. In 1902 vuillemin grouped the species described for this genus under the trichosporon beigelii denomination, a practice that remained until the late 90s. A 67 year old male presented with a widespread erythematous rash prior to sustaining a syncopal episode outside the dermatologistsof. Both can be a constituent of the normal microbiota but can also cause both superficial and invasive infections in humans. Trichosporon asahii among intensive care unit patients at a. Trichosporon asahii taking a step back, these are the t. Trichosporon genus is subdivided into six distinct human pathogenic species of which t. Draft genome sequence of cbs 2479, the standard type.
We describe a 55yearold man with acute myelogenous leukemia who developed breakthrough trichosporon asahii fungemia during 5 days of micafungin treatment. Trichosporon asahii, a non candida yeast that caused. A case of invasive trichosporonosis due to trichosporon asahii in an otherwise healthy young adult male presenting as meningoencephalitis and pneumonia is reported here. Infeccion sistemica por trichosporon asahii request pdf. Following the results of dnabased identification, trichosporon asahii was the species most frequently isolated from deep sites 15 of 25 strains. It is this form that makes trichosporon a yeastlike fungus.
Bloodstream infections due to trichosporon spp species distribution, trichosporon asahii genotypes determined on the basis of ribosomal dna intergenic spacer 1. The first reported case of trichosporon asahii in systemic. Trichosporon trikosporon, trikosporon, a genus of imperfect fungi that possess branching septate hyphae with arthroconidia and blastoconidia. Free cells are seen to the left with the edge of the colony to the right. Most are typically isolated from soil, but several species occur as a natural part of the skin. Trichosporonosis is an emerging infection predominantly caused by trichosporon asahii which is a ubiquitous and exclusively anamorphic yeast. Sequencing of the ribosomal dna rdna intergenic spacer 1 igs1 is currently mandatory for accurate trichosporon identification, but it is not usually performed in routine laboratories. It is ubiquitous, found in soil, vegetation and water samples. Catheterrelated trichosporon asahii bloodstream infection. It is a yeastlike fungus, often found in soil, decomposing wood, air, rivers, lakes, seawater, cheese, scarab beetles, bird droppings, bats, pigeons and cattle. Trichosporon asahii is a ureasepositive, nonencapsulated basidiomycetous yeast. Trichosporon asahii was identified from blood cultures, confirmed by morphological examination on cornmeal agar and speciated by biochemical analysis api 20c. Jul 01, 2009 invasive trichosporonosis caused by trichosporon asahii and other unusual trichosporon species at a medical.
Oct 17, 2016 trichosporon asahii was the main causative species 46. Trichosporon asahii infection in an advanced aids patient. The name trichosporon cutaneum is often used synonymously with trichosporon beigelii. Granuloma associated with trichosporon asahii infection in the lung. Trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen seen particularly in immunologically compromised patients. Suppurative flexor tenosynovitis from tsumakurella and. Trichosporon asahii among intensive care unit patients at a medical center in jamaica volume 34 issue 6 robyn neblett fanfair, orville heslop, kizee etienne, lois rainford, monika roy, lalitha gade, joyce peterson, heather oconnell, judith noblewang, s. Original article by the brazilian journal of infectious diseases. Conventional methods of its detection are timeconsuming or costly and often require complex dna extraction and purification steps, which hinders rapid clinical diagnosis. Trichosporon asahii is a pathogenic basidiomycetous yeast. Trichosporon asahii is an emerging cause of systemic fungal infection in an immunocompromised host. The most common serotype ii species isolated from the homes of shp patients is t. Trichosporon is a genus of anamorphic fungi in the family trichosporonaceae.
Urinary tract infection by trichosporon asahii sood s, pathak. Trichosporon grows as a yeast, both at room temperature and at 37. First two cases in chile we present two cases of nosocomial urinary tract infection due to trichosporon asahii in intensive care unit patients with bladder catheter from two hospitals in. Trichosporon asahii, infeccion urinaria nosocomial, sensibilidad antifungica, patogeno emergente nosocomial urinary infection due to trichosporon asahii. Its resistance to certain antifungal agents makes it difficult to treat. However, it is not clear whether cell surface phenotypes differ among the colony morphologies.
A fatal outbreak of trichosporon asahii sepsis in a neonatal intensive care unit. Two case presentations infected by trichosporon asahii and. Trichosporonosis definition of trichosporonosis by. Furthermore, four genotypes were identified in tunisia. Here, we first report the draft nuclear chromosome genome sequence and mitochondrial genome sequence of t. This case represents a probable infection by trichosporon, since it occurred in a patient with neutropenic fever and the organism.
An trichosporon asahii in nahilalakip ha genus nga trichosporon, ngan familia nga trichosporonaceae. The patient was treated with amphotericin b and fluconazole, but t. Trichosporon asteroides is an asexual basidiomycetous fungus first described from human skin but is now mainly isolated from blood and urine. In vitro antifungal susceptibility testing of the isolates showed that voriconazole exhibited the highest activity. Disseminated trichosporon asahii infection in a combined. Pcr then showed trichosporon species and later the blood culture grew yeast for which caspofungin was added. October 2006 sood et al urinary tract infection by t. Sep 28, 2019 trichosporon is a genus of anamorphic fungi in the family trichosporonaceae.
However, igs1 sequencing unambiguously identified all trichosporon isolates. Feb 12, 2018 a fatal outbreak of trichosporon asahii sepsis in a neonatal intensive care unit. Bloodstream infections due to trichosporon spp species. The reevaluation of the genus trichosporon has led to the replacement of the old taxon trichosporon beigelii by six new species. This pdf is available for free download from a site hosted by medknow publications. Trichosporon asahii trichosporon beigelii infections are rare but have been associated with a wide spectrum of clinical manifestations, ranging from superficial involvement in immunocompetent individuals to severe systemic disease in immunocompromised.
Trichosporon asahii is a basidiomycetous yeast which causes white piedra and onychomycosis in. Trichosporon asahii is not only the causative agent of trichosporonosis, but also causes shp representing type iii or iv allergy, which may develop after repeated inhalation of arthroconidia. In 1902, vuillemin designated all trichosporon species trichosporon beigelii, an arthrosporecontaining yeast 66, 69. Clinical breakpoints have not been defined for trichosporon species, and the mics cannot be reliably interpreted as indicating resistance or susceptibility. Successful treatment of trichosporon asahii fungemia with. Invasive trichosporonosis caused by trichosporon asahii and. Susceptibility patterns and molecular identification of.
Trichosporon species and blastoschizomyces capitatus. Invasive trichosporonosis due to trichosporon asahii in a non. Feb 12, 2018 trichosporon species are fungi that commonly inhabit the soil. Rapid and simple detection of trichosporon asahii by.
In this study, we evaluated colony pcr, which directly uses colonies or trace clinical samples as the template for amplification, for rapid. Trichosporon definition of trichosporon by medical dictionary. They colonize the skin and gastrointestinal tract of humans. The first reported case of trichosporon asahii in systemic lupus erythematosus sle from riyadh, saudi arabia albaadani a1, kallam k1, albarrag a2 1department of medicine, prince sultan military medical city, riyadh, saudi arabia. Trichosporon asahii is the most frequently isolated species in invasive trichosporonosis 2. T asahii is also well known to be responsible for causing white piedra and onychomycosis in immunocompetent patients 10. Trichosporon species and blastoschizomyces capitatus are related fungi and are rare causes of invasive infection in humans.
Disseminated, fatal trichosporon asahii infection in a bone marrow transplant recipient. Although the patients clinical condition improved considerably after the start of voriconazole treatment, blood culture results remained positive for t. Multidrugresistant trichosporon asahii infection of. In the main, other trichosporon species were recovered from cutaneous samples. First genotype identification of trichosporon asahii in sfax. Production of monoclonal antibodies specific to the human. Trichosporon spp an emerging fungal pathogen medicina. All patients had central venous catheter and received prior antibiotics. This pdf is available for free download from a site hosted. It was identified as trichosporon asahii using standard techniques.